Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

181 – 195 of 3,302 results

181

Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 8.0

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

182

Thermo Scientific Chemicals MES, 1.0M buffer soln., pH 5.5

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00283848 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O

Pricing & Availability
Specifications

PubChem CID	16218417
CAS	145224-94-8
Molecular Weight (g/mol)	213.248
MDL Number	MFCD00283848
SMILES	C1COCCN1CCS(=O)(=O)O.O
IUPAC Name	2-morpholin-4-ylethanesulfonic acid;hydrate
InChI Key	MIIIXQJBDGSIKL-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

183

Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 10X), pH 7.6

Helps to maintain a constant pH

Pricing & Availability
Specifications

Physical Form	Liquid
pH	7.6
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Recommended Storage	Ambient temperatures
Concentration	10 X

184

MP Biomedicals™ Phosphate Buffered Saline Tablets (PBS)

Phosphate Buffered Saline Tablets (PBS) are a balanced salt mixture for easy PBS buffer preparation for a variety of applications, including reagent preparation, diluting cells for flow cytometry, and cell culture reagent.

Pricing & Availability
Specifications

Includes	No Calcium, No Magnesium
Molecular Weight (g/mol)	74.55
MDL Number	MFCD00011360
Physical Form	Tablet
Chemical Name or Material	Phosphate Buffered Saline (PBS)
SMILES	[Cl-].[K+]
IUPAC Name	potassium chloride
InChI Key	WCUXLLCKKVVCTQ-UHFFFAOYSA-M
Molecular Formula	ClK

185

Thermo Scientific Chemicals Tricine, 98%, for biochemistry

CAS: 4-1-5704 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 MDL Number: MFCD00004277 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N Synonym: N-[Tris(hydroxymethyl)methyl]glycine,N-(2-Hydroxy-1, 1-bis(hydroxymethyl)ethyl)glycine PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO

Pricing & Availability
Specifications

PubChem CID	79784
CAS	4-1-5704
Molecular Weight (g/mol)	179.17
ChEBI	CHEBI:39063
MDL Number	MFCD00004277
SMILES	C(C(=O)O)NC(CO)(CO)CO
Synonym	N-[Tris(hydroxymethyl)methyl]glycine,N-(2-Hydroxy-1, 1-bis(hydroxymethyl)ethyl)glycine
IUPAC Name	2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid
InChI Key	SEQKRHFRPICQDD-UHFFFAOYSA-N
Molecular Formula	C6H13NO5

186

Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 8.0, 0.2 micron filtered

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

187

Thermo Scientific Chemicals ACES Buffer (0.5 M, pH 7.0)

CAS: 7365-82-4 Molecular Formula: C4H10N2O4S Molecular Weight (g/mol): 182.194 MDL Number: MFCD00008030 InChI Key: DBXNUXBLKRLWFA-UHFFFAOYSA-N PubChem CID: 81832 ChEBI: CHEBI:39062 IUPAC Name: 2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid SMILES: C(CS(=O)(=O)O)NCC(=O)N

Pricing & Availability
Specifications

PubChem CID	81832
CAS	7365-82-4
Molecular Weight (g/mol)	182.194
ChEBI	CHEBI:39062
MDL Number	MFCD00008030
SMILES	C(CS(=O)(=O)O)NCC(=O)N
IUPAC Name	2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid
InChI Key	DBXNUXBLKRLWFA-UHFFFAOYSA-N
Molecular Formula	C4H10N2O4S

188

Thermo Scientific Chemicals PIPES, 1.0M buffer soln., pH 7.0

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

Pricing & Availability
Specifications

PubChem CID	79723
CAS	5625-37-6
Molecular Weight (g/mol)	302.36
ChEBI	CHEBI:44933
MDL Number	MFCD00006159
SMILES	C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
IUPAC Name	2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	IHPYMWDTONKSCO-UHFFFAOYSA-N
Molecular Formula	C8H18N2O6S2

189

MOPS, MP Biomedicals™

CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N Synonym: 3-(N-morpholino)propanesulfonic acid,4-morpholinepropanesulfonic acid PubChem CID: 70807 ChEBI: CHEBI:44115 SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1

Pricing & Availability
Specifications

PubChem CID	70807
CAS	1132-61-2
Molecular Weight (g/mol)	209.26
ChEBI	CHEBI:44115
MDL Number	MFCD00006183
SMILES	[O-]S(=O)(=O)CCC[NH+]1CCOCC1
Synonym	3-(N-morpholino)propanesulfonic acid,4-morpholinepropanesulfonic acid
InChI Key	DVLFYONBTKHTER-UHFFFAOYSA-N
Molecular Formula	C7H15NO4S

190

Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 6.8

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

191

Tricine, Electrophoresis Grade, 99%

CAS: 4-1-5704 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 MDL Number: MFCD00004277 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N Synonym: N-(Trishydroxymethylmethyl)glycine PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO

Pricing & Availability
Specifications

PubChem CID	79784
CAS	4-1-5704
Molecular Weight (g/mol)	179.17
ChEBI	CHEBI:39063
MDL Number	MFCD00004277
SMILES	C(C(=O)O)NC(CO)(CO)CO
Synonym	N-(Trishydroxymethylmethyl)glycine
IUPAC Name	2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid
InChI Key	SEQKRHFRPICQDD-UHFFFAOYSA-N
Molecular Formula	C6H13NO5

192

Thermo Scientific Chemicals MES, 99+%, <1% water

CAS: 4432-31-9 Molecular Formula: C6H13NO4S Molecular Weight (g/mol): 195.233 MDL Number: MFCD00006181 InChI Key: SXGZJKUKBWWHRA-UHFFFAOYSA-N Synonym: 2-(N-Morpholino)ethanesulfonic acid PubChem CID: 78165 ChEBI: CHEBI:39005 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid SMILES: C1COCCN1CCS(=O)(=O)O

Pricing & Availability
Specifications

PubChem CID	78165
CAS	4432-31-9
Molecular Weight (g/mol)	195.233
ChEBI	CHEBI:39005
MDL Number	MFCD00006181
SMILES	C1COCCN1CCS(=O)(=O)O
Synonym	2-(N-Morpholino)ethanesulfonic acid
IUPAC Name	2-morpholin-4-ylethanesulfonic acid
InChI Key	SXGZJKUKBWWHRA-UHFFFAOYSA-N
Molecular Formula	C6H13NO4S

193

Thermo Scientific Chemicals TRIS, 0.5M buffer soln., pH 6.8

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

194

Thermo Scientific Chemicals HEPES, 1.0M buffer soln., pH 7.0

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

Pricing & Availability
Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
IUPAC Name	2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

195

Thermo Scientific Chemicals PIPES, 0.5M buffer soln., pH 6.5

Pricing & Availability
Specifications

PubChem CID	79723
CAS	5625-37-6
Molecular Weight (g/mol)	302.36
ChEBI	CHEBI:44933
MDL Number	MFCD00006159
SMILES	C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
IUPAC Name	2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	IHPYMWDTONKSCO-UHFFFAOYSA-N
Molecular Formula	C8H18N2O6S2

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